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AS 4276.3:2021
[Current]Water microbiology, Method 3: Enumeration of heterotrophic microorganisms — Pour plate, spread plate, membrane filtration and most probable number techniques
Establishes a standard method for the examination of water, with or without biocides, for the enumeration of heterotrophic microorganisms that covers a choice of possible techniques. KEYWORDS: Water microbiology; Enumeration; Heterotrophic microorganisms
Published: 05/03/2021
Pages: 17
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Introduction
1 Scope and general
1.1 Scope
1.2 Normative references
1.3 Terms and definitions
2 Principle
3 Samples
3.1 Laboratory samples
3.2 Dilutions
4 Apparatus
5 Culture media and diluents
6 Quality assurance
6.1 General
6.2 Performance testing of culture media
6.3 Reference cultures
6.3.1 General
6.3.2 Positive culture
7 Techniques
7.1 Pour plate
7.1.1 General
7.1.2 Procedure
7.1.3 Inoculation and pouring of plates
7.1.4 Incubation
7.1.5 Counting of colonies
7.1.6 Calculation of results
7.2 Spread plate
7.2.1 General
7.2.2 Procedure
7.2.3 Incubation
7.2.4 Counting of colonies
7.2.5 Calculation of results
7.3 Spiral plate
7.3.1 General
7.3.2 Procedure
7.3.3 Incubation
7.3.4 Reading of results
7.3.5 Calculation of results
7.4 Membrane filtration
7.4.1 General
7.4.2 Procedure
7.4.3 Incubation
7.4.4 Counting of colonies
7.4.5 Calculation of results
7.5 Most probable number (MPN)
7.5.1 General
7.5.2 Procedure
7.5.3 Reading of results
7.5.4 Calculation of results
8 Test report
Appendix A
Appendix B
B.1 Scope
B.2 Reasoner’s 2A agar (R2A)
B.2.1 Reference
B.2.2 Materials
B.2.3 Apparatus
B.2.4 Procedure
B.3 Plate count agar (PCA)
B.3.1 Reference
B.3.2 Materials
B.3.3 Apparatus
B.3.4 Procedure
B.4 m-HPC agar
B.4.1 Reference
B.4.2 Materials
B.4.3 Apparatus
B.4.4 Procedure
B.5 Yeast extract agar (YEA)
B.5.1 Reference
B.5.2 Materials
B.5.3 Apparatus
B.5.4 Procedure
B.6 Tryptone soy/soya agar (casein soya bean digest agar) (TSA)
B.6.1 Reference
B.6.2 Materials
B.6.3 Apparatus
B.6.4 Procedure
B.7 Nutrient agar
B.7.1 Reference
B.7.2 Materials
B.7.3 Apparatus
B.7.4 Procedure
Bibliography
Cited references in this standard
[Current]
Water microbiology, Method 1: Water quality — General requirements and guidance for microbiological examinations by culture (ISO 8199:2018, MOD)
[Current]
Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media (ISO 11133:2014, MOD)
[Pending Revision]
Water quality — Sampling for microbiological analysis (ISO 19458:2006, MOD)
Content history
[Superseded]
[Superseded]
DR AS 4276.3:2020
AS/NZS 4276.3.2:2003 Rec:2013
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