Standard
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AS/NZS 4276.3.2:2003
[Superseded]Water microbiology, Method 3.2: Heterotrophic colony count methods — Plate count of water containing biocides
This Standard sets out a method for estimating the number of colony-forming units (CFUs) in water using a pour plate technique and R2A Agar. For waters which may contain growth-inhibiting additives which have not been neutralized, plate counts starting with the 1:100 dilution are recommended in order to eliminate interference with counts. This increases the lower detection limit of the method to 100 cfu/mL. This limit is generally adequate for applications such as the monitoring of cooling tower water.
Published: 18/03/2003
Pages: 4
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Foreword
1 Scope
2 Referenced documents
3 Culture medium
4 Apparatus
4.1 Apparatus for preparation of dilutions— see AS 4276.1.
4.2 Petri dishes—about 90 mm diameter.
4.3 Pipettes or equivalent measuring devices — 1 mL.
4.4 Colony counter
4.5 Tally counter
4.6 Incubators or water baths
5 Samples
5.1 Laboratory samples
5.2 Dilutions
6 Procedure
6.1 General
6.2 Preparation of dilutions
6.3 Inoculation and pouring of plates
6.4 Incubation
6.5 Counting of colonies
7 Calculation
8 Test report
Appendix A
A1 Scope
A2 R2A Agar
Cited references in this standard
[Current]
Water microbiology, Method 1: Water quality — General requirements and guidance for microbiological examinations by culture (ISO 8199:2018, MOD)
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