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AS 5140:2022
[Current]Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media (ISO 11133:2014, MOD)
AS 5140:2022 adopts and modifies ISO 11133:2014, and its Amendments No. 1 (2018) and 2 (2020) for Australia, defining terms related to quality assurance of culture media and specifying requirements for the preparation of culture media intended for the microbiological analysis of food, animal feed, and samples from the food or feed production environment as well as water intended for consumption or used in food production.
Published: 02/12/2022
Pages: 141
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
3.1 General terms and definitions
3.2 Terminology of performance testing
3.3 Terminology of culture media
3.4 Terminology for test microorganisms
4 Quality assurance management
4.1 Documentation
4.1.1 Documentation from manufacturer or producer
4.1.2 Delivery acceptance of products
4.2 Storage
4.2.1 General
4.2.2 Quality management and product control of dehydrated media and supplements
4.3 Laboratory preparation of media
4.3.1 General
4.3.2 Quality of basic medium components
4.3.3 Water
4.3.4 Weighing and rehydration
4.3.5 Dissolution and dispersion
4.3.6 Measurement and adjustment of pH
4.3.7 Dispensing
4.3.8 Sterilization
4.3.8.1 General
4.3.8.2 Sterilization by moist heat
4.3.8.3 Sterilization by filtration
4.3.9 Preparation of supplements
4.4 Storage and shelf-life of prepared media
4.4.1 Commercially supplied media
4.4.2 Laboratory prepared media
4.4.2.1 General
4.4.2.2 Shelf-life evaluation
4.4.2.3 Storage of media in Petri dishes
4.5 Preparation for use
4.5.1 Melting of agar culture media
4.5.2 De-aeration of culture media
4.5.3 Addition of supplements
4.5.4 Preparation of solid media in Petri dishes
4.5.5 Preparation of plated media for inoculation
4.6 Incubation of solid media in Petri dishes
4.7 Disposal of media
5 Test organisms for performance testing
5.1 General
5.2 Selection of test organisms
5.3 Preservation and maintenance of test organisms
5.3.1 General
5.3.2 Test microorganisms from commercial sources
5.3.3 Laboratory prepared reference stocks
5.3.4 Stock cultures
5.3.5 Working cultures
5.4 Microorganisms for performance testing
5.4.1 General
5.4.2 Preparation
5.4.2.1 Preparation of stock cultures
5.4.2.2 Preparation of working cultures
5.4.2.3 Preparation of suspensions (inocula) for the test
5.4.2.4 Volumes of inocula
5.4.2.5 Inoculum level
5.4.2.5.1 Inoculum level for productivity testing
5.4.2.5.1.1 Quantitative testing
5.4.2.5.1.2 Qualitative testing
5.4.2.5.2 Inoculum level for selectivity testing
5.4.2.5.3 Inoculum level for specificity testing
5.4.2.6 Incubation
6 Quality control and performance testing of culture media
6.1 General requirements
6.2 Physical and chemical quality control
6.3 Microbiological quality control
6.3.1 General
6.3.2 Reference medium
6.3.3 Microbial contamination
6.4 General requirements for microbiological performance testing
6.4.1 General
6.4.2 Ready-to-use media
6.4.3 Media prepared from commercially available dehydrated formulations
6.4.4 Media prepared from basic individual components
6.5 Performance evaluation and interpretation of results
6.6 Confirmation media and reagents
6.6.1 Confirmation media
6.6.2 Confirmation reagents
7 Methods for performance testing of solid culture media
7.1 General
7.2 Methods for quantitative tests
7.2.1 Methods for quantitative tests — Definitions
7.2.1.1 Productivity
7.2.1.2 Selectivity
7.2.2 Quantitative method for solid culture media
7.2.2.1 General
7.2.2.1.1 Procedure
7.2.2.1.2 Calculation and interpretation of results
7.2.2.2 Using recovery from reference materials
7.3 Testing of culture media used for membrane filtration
7.4 Methods for qualitative tests
7.4.1 Qualitative streaking method
7.4.1.1 Procedure
7.4.1.2 Interpretation of results
7.4.2 Determination of specificity
7.4.3 Other qualitative methods for solid media
8 Methods for performance testing of liquid culture media
8.1 General
8.2 Quantitative tube method for performance testing of liquid enrichment media (dilution to extinction method)
8.2.1 General
8.2.2 Preparation of the dilution series
8.2.3 Procedure for testing the liquid medium
8.2.4 Calculation and interpretation of results
8.3 Qualitative tube method for performance testing of selective liquid media
8.3.1 General
8.3.2 Procedure
8.3.3 Calculation and interpretation of results
8.4 Qualitative single tube method (turbidity) for performance testing of liquid media
8.4.1 General
8.4.2 Procedure
8.4.2.1 Pre-enrichment media
8.4.2.2 Confirmation media
8.4.3 Interpretation of results
9 Methods for performance testing of diluents and transport media
9.1 General
9.2 Method for testing diluents
9.2.1 Method for quantitative testing of diluents
9.2.1.1 General
9.2.1.2 Procedure
9.2.1.3 Reading and interpretation of results
9.3 Method for testing transport media
9.3.1 General
9.3.2 Method for quantitative testing of liquid transport media
9.3.2.1 Procedure
9.3.2.2 Reading and interpretation of results
9.3.3 Method for qualitative testing of solid transport media
9.3.3.1 Procedure
9.3.3.2 Reading and interpretation of results
10 Documentation of test results
10.1 Information provided by the manufacturer
10.2 Traceability
Annex A
A.1 General
A.2 Peptones
A.3 Extracts and infusions
A.4 Agar
A.5 Other
Annex B
B.1 Preparation of reference stock from a reference strain
B.2 Preparation of working culture from reference stock
Annex C
C.1 General
C.2 Quantitative method for solid culture media: productivity and selectivity (see 7.2.2 and Figure C.1)
C.3 Quantitative tube method for performance testing of liquid enrichment media — Dilution to extinction method (see 8.2 and Figure C.2)
C.4 Qualitative single tube method for selective liquid enrichment media (with target, non-target, or a mixture of target and non-target microorganisms in the same tube) (see 8.3 and Figure C.3)
C.5 Qualitative single tube method for non-selective and selective liquid media: turbidity (see 8.4 and Figure C.4)
Annex D
Annex E
Annex F
Annex G
G.1 General
G.2 Using control charts
G.2.1 General procedure
G.2.2 Production of a control chart
G.2.3 Worked example of producing a control chart using 20 results
G.2.4 Performance evaluation and interpretation of results
G.2.5 Alternative approaches to monitoring media performance
G.2.6 Periodic review of control charts
Annex H
Annex I
I.1 General
I.2 Method for quantitative testing of non-selective liquid culture media using target microorganisms
I.2.1 Procedure
I.2.2 Counting and interpretation of results
I.2.3 Flow chart for quantitative method for non-selective liquid culture media using target microorganisms
I.3 Method for quantitative testing of selective liquid culture media using target and non-target microorganisms
I.3.1 Procedure
I.3.2 Reading, calculation and interpretation of results
I.3.3 Flow chart for quantitative method for selective liquid culture media using target and non-target microorganisms
Annex J
J.1 General
J.2 General
J.3 Performance criteria, methods and targets
J.4 Choice of performance control strains
J.4.1 General
J.4.2 Assessment of suitability of new control strains
J.4.3 New media
J.4.4 Number of strains per criterion
Bibliography
Amendment 1
Amendment 2
Annex K
Appendix ZZ
ZZ.1 Scope
ZZ.2 Variations
Cited references in this standard
[Current]
Water quality - General requirements and guidance for microbiological examinations by culture
[Current]
Microbiology of the food chain - General requirements and guidance for microbiological examinations
[Current]
Water quality - Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods
[Current]
Microbiology of food and animal feed - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 6: Specific rules for the preparation of samples taken at the primary production stage
[Current]
Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 5: Specific rules for the preparation of milk and milk products
Content history
[Superseded]
[Current]
[Current]
[Current]
DR AS 5140:2022
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