Standard
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AS/NZS 4276.6:2007
[Current]Water microbiology, Method 6: Coliforms, Escherichia coli and thermotolerant coliforms — Determination of most probable number (MPN)
This Standard sets out a method, using a multiple tube dilution technique, for determining the most probable number (MPN) of coliforms, Escherichia coli (E. coli) and thermotolerant coliforms in water. It is suitable for waters with high background flora and/or turbidity.
Published: 20/03/2007
Pages: 11
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
1 Scope
2 Referenced documents
3 Definitions
4 Principle
5 Culture media and reagents (See Appendix B)
5.1 Improved formate lactose glutamate (IFLG) medium
5.2 MacConkey agar (MA)
5.3 Ortho-nitrophenyl-β-galactopyranoside (ONPG) broth
5.4 Nutrient agar
5.5 Oxidase reagent (Kovacs')
5.6 EC-broth with 4-methylumbelliferyl-β-D-glucuronide with fermentation tube (EC-MUG)
6 Apparatus
6.1 Rimless bacteriological test tubes or containers of appropriate size
6.2 Pipettes or equivalent measuring devices
6.3 Fermentation tubes (Durham’s)
6.4 Incubators or water baths
6.5 Long wave ultra-violet light (approximately 360 nm)
7 Reference cultures
7.1 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803 or NZRM 3309 or a culture traceable to any of these strains
7.2 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or NZRM 798 or a culture traceable to any of these strains
7.3 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
7.4 Use of reference cultures
8 Samples
8.1 Laboratory samples
8.2 Dilutions
9 Procedure
9.1 Presumptive test
9.2 Confirmatory tests
9.2.1 Coliforms
9.2.2 E. coli
9.2.3 Thermotolerant coliforms
10 Determination of the most probable number (MPN) of coliforms, E. coli and thermotolerant coliforms
11 Test report
12 Bibliography
Appendix A
Appendix B
B1 Scope
B2 Improved formate lactose glutamate medium
B3 Ortho-nitrophenyl-β D-galactopyranoside (ONPG) broth
B4 Nutrient agar
B5 MacConkey agar
B6 Oxidase reagent (Kovacs’)
B7 EC-MUG broth
Appendix C
Cited references in this standard
[Superseded]
Water microbiology, Method 1: General information and procedures (ISO 8199:2005, MOD)
Content history
[Superseded]
[Superseded]
AS/NZS 4276.6-2007 REC:2018
DR 06120
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