Standard
UPDATE AVAILABLE
AS 5013.4-2009
[Superseded]This standard gives general guidelines for the enumeration of coliforms.
Published: 18/02/2009
Pages: 6
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1
4.2
4.3
4.4
5 Culture media and diluents
5.1 General
5.2 Diluents
5.3 Solid selective medium: Crystal violet neutral red bile lactose (VRBL) agar
5.3.1 Composition
5.3.2 Preparation
5.3.3 Performance testing for the quality assurance of the culture medium
5.4 Confirmation medium: Brilliant green lactose bile broth
5.4.1 Composition
5.4.2 Preparation
6 Apparatus and glassware
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.2.1
9.2.2
9.2.3
9.2.4
9.3 Enumeration
9.4 Confirmation
10 Expression of results
11 Precision
12 Test report
Bibliography
Cited references in this standard