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AS 5013.4:2021
[Current]Food microbiology, Method 4: Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coliforms — Colony-count technique (ISO 4832:2006, MOD)
AS 5013.4 adopts and modifies ISO 4832:2006 for Australia, setting out general guidelines for the enumeration of coliforms. Applicable to: (a) products intended for human consumption and for the feeding of animals; and (b) environmental samples in the area of food production and food handling, by means of the technique of counting colonies after incubation on a solid medium at 30 °C or at 37 °C.
Published: 10/12/2021
Pages: 8
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1
4.2
4.3
4.4
5 Culture media and diluents
5.1 General
5.2 Diluents
5.3 Solid selective medium: Crystal violet neutral red bile lactose (VRBL) agar
5.3.1 Composition
5.3.2 Preparation
5.3.3 Performance testing for the quality assurance of the culture medium
5.4 Confirmation medium: Brilliant green lactose bile broth
5.4.1 Composition
5.4.2 Preparation
6 Apparatus and glassware
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.2.1
9.2.2
9.2.3
9.2.4
9.3 Enumeration
9.4 Confirmation
10 Expression of results
11 Precision
12 Test report
Bibliography
Appendix ZZ
ZZ.1 Scope
ZZ.2 Variations
Cited references in this standard
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