Standard
UPDATE AVAILABLE
AS/NZS 4276.19:2001
[Superseded]Water microbiology, Method 19: Examination for thermophilic Campylobacter spp.- Membrane filtration
This Standard sets out a method for detecting thermophilic Campylobacters in water using culture in enrichment broth and on selective agar media.
Published: 24/07/2001
Pages: 9
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
1 Scope
2 Referenced document
3 Principle
4 Culture media, reagents and reference cultures
4.1 Culture media (See Appendix B)
4.1.1 Preston enrichment broth
4.1.2 Prestons selective agar or a blood free modification of this medium
4.1.3 Blood agar
4.2 Reagent
4.3 Reference cultures
4.3.1 Campylobacter jejuniNCTC 11351 or ACM 3393 or NZRM 2397. This organism gives positive reactions for thermophilic Campylobacter spp.
4.3.2 EscherichiacoliNCTC 9001 or ATCC 11775 or ACM 1803 or NZRM 3309. This organism gives negative reactions for thermophilic Campylobacter spp.
4.3.3 Use of reference cultures
5 Apparatus
5.1 Gas cylinders or kits
5.2 Filtration equipment and 0.2 μm or 0.45 μm membranes
5.3 Incubators
6 Procedures
6.1 Micro-aerobic incubation conditions
6.2 Sample volume
6.3 Membrane filtration
6.4 Enrichment
6.5 Controls for broth culture
6.6 Selective enrichment
6.7 Plating out on selective agar media
6.8 Examination of selective agar plates
7 Confirmation tests
7.1 Culture of selected colonies
7.2 Oxidase test and Gram stain
7.3 Further confirmation tests
8 Test report
9 Bibliography
Appendix A
Appendix B
B1 Scope
B2 Blood agar
B2.1 General
B2.2 Nutrient agar
B3 Preston enrichment broth
B3.1 General
B3.2 Nutrient broth
B3.3 FBP Supplement
B3.4 Antibiotic supplement
B4 Preston selective agar
B5 Campylobacter blood-free selective medium (modified CCDA-prestons)
B6 Carbol fuchsin
B6.1 Strong
B6.2 Weak
Cited references in this standard
AS
4276.1
Water microbiology, Method 1: General information and procedures
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