Standard
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AS/NZS 4276.17.2:2000
[Superseded]Water microbiology, Method 17.2: Spores of sulfite-reducing anaerobes (clostridia) including Clostridium perfringens — Estimation of most probable number (MPN) using the multiple tube dilution technique
This Standard sets out a method for determining the most probable number (MPN) of spores of sulfite-reducing anaerobes (clostridia) including C.perfringens in water, using a multiple
tube dilution technique, with a confirmation procedure for identifying C.perfringens.
Published: 20/11/2000
Pages: 8
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
1 Scope
2 Referenced documents
3 Principle
4 Media, reagents, and reference cultures
4.1 Culture media (see AS 4276.2 and Appendix B)
4.1.1 Differential reinforced clostridial medium (DRCM)
4.1.2 Tryptose sulfite cycloserine agar (without egg yolk) (TSC)
4.1.3 Lactose gelatine medium (LG)
4.1.4 Nitrate motility medium (NM)
4.2 Nitrite detection reagents (see Appendix B)
4.2.1 Solution A
4.2.2 Solution B
4.3 Reference culture
4.3.1 Positive culture
4.3.2 Negative culture
4.3.3 Use of reference culture
5 Apparatus
5.1 Suitable screw cap containers for the volume of water to be tested
5.2
5.3
6 Test procedures
6.1 MPN by multiple tube dilution technique
7 Confirmation of C.perfringens
8 Report
9 Bibliography
Appendix A
Appendix B
B1 Scope
B2 Differential reinforced clostridial medium (single strength)
B3 Tryptose sulfite cycloserine agar
B4 Lactose gelatin medium
B5 Nitrate motility medium
B6 Nitrite detection reagents
B6.1 Solution A, Sulfanilic acid reagent
B6.2 Solution B, N-(1-naphthyl)ethylenediamine reagent
Cited references in this standard
[Current]
Water microbiology, Method 1: Water quality — General requirements and guidance for microbiological examinations by culture (ISO 8199:2018, MOD)
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