Standard
UPDATE AVAILABLE
AS/NZS 4276.15:1999
[Superseded]Water microbiology, Method 15: Examination for Vibrio cholerae
This Standard sets out a method for detecting Vibrio cholerae in water using culture in
enrichment broth and on selective agar media.
Published: 05/10/1999
Pages: 14
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
PREFACE
1 SCOPE
2 PRINCIPLE
3 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
3.1 Culture media
3.1.1 Alkaline peptone water (APW)
3.1.2 Thiosulphate citrate bile salt sucrose agar (TCBS)
3.1.3 Nutrient agar
3.1.4 3% NaCl tryptone water (3% NaCl TW)
3.1.5 0% NaCl tryptone water (0% NaCl TW)
3.1.6 Tryptone water
3.1.7 Buffered glucose broth
3.1.8 Arginine dihydrolase broth
3.1.9 Lysine decarboxylase broth
3.1.10 Ornithine decarboxylase broth
3.1.11 Control decarboxylase broth
3.1.12 Glucose fermentation broth
3.1.13 Arabinose fermentation broth
3.1.14 Cellobiose fermentation broth
3.1.15 Lactose fermentation broth
3.1.16 Maltose fermentation broth
3.1.17 Mannitol fermentation broth
3.1.18 Salicin fermentation broth
3.1.19 Sucrose fermentation broth
3.1.20 Nitrate reduction broth
3.1.21 O-nitrophenyl-β-D-galactopyranoside broth (ONPG)
3.1.22 8% NaCl tryptone water
3.2 Reagents
3.2.1 Kovacs’ oxidase reagent
3.2.2 Kovacs’ indole reagent
3.2.3 5% alpha naphthol in ethanol
3.2.4 40% KOH
3.2.5 Nitrate reagent A (0.8% w/v sulphanilic acid in 5N acetic acid)
3.2.6 Nitrate reagent B (0.6% v/v N,N - Dimethly-1-naphthylamine in 5N acetic acid)
3.3 Reference cultures
3.3.1 Positive culture
3.3.2 Negative culture
3.3.3 Use of reference cultures
4 SAMPLES
4.1 Sample volume
4.2 Concentration techniques
4.2.1 Moore swab
4.2.2 Membrane filtration
5 PROCEDURE
5.1 Enrichment
5.2 Selective agar cultures
5.3 Presumptive identification of Vibrio cholerae
5.4 Confirmation of Vibrio cholerae
6 TEST REPORT
7 ENUMERATION PROCEDURES
8 BIBLIOGRAPHY
APPENDIX A
APPENDIX B
B1 SCOPE
B2 0%, 3%, 8% NaCl TRYPTONE WATER
B3 ALKALINE PEPTONE WATER
B4 BUFFERED GLUCOSE BROTH
B5 CARBOHYDRATE FERMENTATION BROTHS
B5.1 Preparation of complete medium
B5.2 Fermentation Broth base
B5.3 Andrade’s indicator
B6 DECARBOXYLASE BROTH (Moeller’s)
B6.1 Preparation of complete medium
B6.2 Decarboxylase broth base (Moeller’s)
B7 NITRATE REDUCTION BROTH
B8 THIOSULFATE CITRATE BILE SALTS SUCROSE AGAR
B9 TRYPTONE WATER
B10 ONPG BROTH
B10.1 Preparation of complete medium
B10.2 Preparation of ONPG solution
APPENDIX C
C1 INDOLE TEST
C2 VOGES-PROSKAUER TEST (FOR THE PRODUCTION OF ACETOIN)
C3 NITRATE REDUCTION TEST (Barritt)
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