Standard
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AS 5013.5:2016
[Current]Food microbiology, Method 5: Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Colony count at 30°C by the pour plate technique
Specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C.
Published: 30/11/2016
Pages: 9
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and diluents
5.1 General
5.2 Diluents
5.3 Agar medium: plate count agar (PCA)
5.3.1 Composition
5.3.2 Preparation
5.3.3 Performance testing of the culture medium
5.3.3.1 General
5.3.3.2 Productivity
5.4 Overlay medium (if necessary; see 9.2.7)
5.4.1 Composition
5.4.2 Preparation
6 Apparatus
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.2.1
9.2.2
9.2.3
9.2.4
9.2.5
9.2.6
9.2.7
9.2.8
9.3 Counting of colonies
9.3.1
9.3.2
10 Expression of results
10.1 Method of calculation
10.2 Precision
10.2.1 General
10.2.2 Repeatability
10.2.3 Reproducibility
10.3 Interpretation of test results
10.3.1 General
10.3.2 Repeatability conditions
10.3.3 Reproducibility conditions
11 Test report
Annex A
A.1 General
A.2 Reproducibility conditions
A.3 Comparison with a limit (one-sided test)
Bibliography
Cited references in this standard
[Current]
Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media
[Current]
Microbiology of the food chain - General requirements and guidance for microbiological examinations
ISO 6887 (all parts)
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination
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