Standard
UPDATE AVAILABLE
AS 5013.3-2009
[Superseded]Food microbiology, Method 3: Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of coliforms — Most probable number technique
Thisl Standard gives general guidelines for the detection and the enumeration of coliforms.
Published: 18/02/2009
Pages: 11
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Detection of coliforms
4.1.1
4.1.2
4.1.3
4.2 Enumeration by the MPN technique
4.2.1
4.2.2
4.2.3
4.2.4
4.2.5
4.2.6
5 Culture media and diluents
5.1 General
5.2 Diluents
5.3 Selective enrichment medium: Lauryl sulfate tryptose broth
5.3.1 Composition
5.3.2 Preparation
5.3.3 Performance testing for the quality assurance of the culture medium
5.4 Confirmation medium: Brilliant green lactose bile broth
5.4.1 Composition
5.4.2 Preparation
5.4.3 Performance testing for the quality assurance of the culture medium
6 Apparatus and glassware
6.1
6.2
6.3
6.4
6.5
6.6
6.7
7 Sampling
8 Preparation of the test sample
9 Procedure (see Annex A)
9.1 Detection method (see Figure A.1)
9.1.1 Test portion and initial suspension
9.1.2 Inoculation and incubation
9.1.2.1
9.1.2.2
9.1.2.3
9.1.3 Confirmation (see Figure A.3)
9.1.3.1
9.1.3.2
9.1.4 Interpretation (see Figure A.1)
9.2 Enumeration method (MPN) (see Figure A.2)
9.2.1 Test portion, initial suspension and dilutions
9.2.2 Inoculation and incubation
9.2.2.1
9.2.2.2
9.2.2.3
9.2.2.4
9.2.2.5
9.2.2.6
9.2.3 Confirmation (see Figure A.3)
9.2.3.1
9.2.3.2
9.2.4 Interpretation (see Figure A.2)
10 Calculation and expression of results
11 Precision
12 Test report
Annex A
Bibliography
Cited references in this standard
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