Standard
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AS 5013.16-2006
[Current]Food microbiology, Method 16: Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of Clostridium perfringens — Colony-count technique
This Standard describes a horizontal method for the enumeration of viable Clostridium perfringens. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of food production and food handling.
Published: 29/12/2006
Pages: 16
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1
4.2
4.3
4.4
5 Diluent, culture media and reagents
5.1 Diluent
5.2 Sulfite-cycloserine agar (SC)
5.2.1 Base
5.2.1.1 Composition
5.2.1.2 Preparation
5.2.2 d-Cycloserine solution
5.2.2.1 Composition
5.2.2.2 Preparation
5.2.3 Complete medium
5.2.4 Performance testing for the quality assurance of SC medium
5.3 Fluid thioglycollate medium
5.3.1 Composition
5.3.2 Preparation
5.3.3 Performance testing for the quality assurance of thioglycollate broth
5.4 Lactose sulfite medium (LS) (optional)
5.4.1 Base medium
5.4.1.1 Composition
5.4.1.2 Preparation
5.4.2 Disodium disulfite solution
5.4.2.1 Composition
5.4.2.2 Preparation
5.4.3 Ammonium iron(III) citrate solution
5.4.3.1 Composition
5.4.3.2 Preparation
5.4.4 Complete medium
5.5 Nitrate motility medium (optional)
5.5.1 Composition
5.5.2 Preparation
5.6 Nitrite detection reagent (optional)
5.6.1 5-Amino-2-naphthalenesulfonic acid (5-2-ANSA) solution
5.6.2 Sulfanilic acid solution
5.6.3 Preparation of complete reagent
5.7 Zinc dust (optional)
5.8 Lactose-gelatin medium (optional)
5.8.1 Composition
5.8.2 Preparation
6 Apparatus and glassware
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation (poured-plate technique)
9.3 Counting and selection of colonies
9.4 Biochemical confirmation
9.4.1 General
9.4.2 Confirmation technique using the LS medium
9.4.2.1 Inoculation and incubation
9.4.2.2 Interpretation
9.4.3 Confirmation technique using the nitrate motility medium and the lactose-gelatin medium
9.4.3.1 General
9.4.3.2 Inoculation and reading of nitrate motility medium
9.4.3.3 Inoculation and reading of lactose-gelatin medium
9.4.3.4 Interpretation
10 Expression of results
10.1 Method of calculation
10.2 Precision
10.2.1 Interlaboratory test
10.2.2 Repeatability
10.2.3 Reproducibility
11 Test report
Annex A
Bibliography
Cited references in this standard
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