Standard
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AS 5013.15-2006
[Current]Food microbiology, Method 15: Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of presumptive Escherichia coli — Most probable number technique
This Standard gives general guidelines for the detection and enumeration of presumptive Escherichia coli by means of the liquid-medium culture technique.
Published: 29/12/2006
Pages: 13
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Detection method
4.1.1
4.1.2
4.1.3
4.1.4
4.1.5
4.1.6
4.1.7
4.2 Enumeration method
4.2.1
4.2.2
4.2.3
4.2.4
4.2.5
4.2.6
4.2.7
4.2.8
5 Diluent, culture media and reagent
5.1 Diluent
5.2 Selective enrichment medium (lauryl sulfate broth)
5.2.1 Composition
5.2.2 Preparation
5.3 EC broth (selective medium)
5.3.1 Composition
5.3.2 Preparation
5.3.3 Performance testing and quality assurance of the culture medium
5.4 Peptone water, indole free
5.4.1 Composition
5.4.2 Preparation
5.5 Indole reagent (Kovacs' reagent)
5.5.1 Composition
5.5.2 Preparation
6 Apparatus and glassware
6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave)
6.2
6.3
6.4
6.5
6.6
6.7
6.8
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Detection method
9.1.1 Test portion and initial suspension
9.1.2 Incubation of the selective enrichment medium (lauryl sulfate broth)
9.1.3 Inoculation and incubation of the selective medium (EC broth)
9.1.4 Inoculation and incubation of the peptone water
9.1.5 Examination for indole production
9.1.6 Interpretation
9.2 Enumeration method
9.2.1 Test portion, initial suspension and dilutions
9.2.2 Inoculation and incubation of the selective enrichment medium (lauryl sulfate broth)
9.2.2.1
9.2.2.2
9.2.2.3
9.2.2.4
9.2.2.5
9.2.3 Subculturing and incubation of the selective medium (EC broth)
9.2.3.1
9.2.3.2
9.2.4 Inoculation and incubation of the peptone water
9.2.5 Examination for indole production
9.2.6 Interpretation
10 Expression of results
10.1 Detection method
10.2 Enumeration method
11 Test report
Annex A
A.1 MPN calculations using three tubes
A.2 MPN calculations using five tubes
Bibliography
Cited references in this standard
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