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AS 5013.14.3:2024
[Current]Food microbiology, Method 14.3: Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony count at 30°C by the surface plating technique (ISO 4833-2:2013, MOD)
AS 5013.14.3:2024 adopts and modifies ISO 4833 2:2013, which specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies on the surface of a solid medium after aerobic incubation at 30 °C. This method is applicable to products intended for human consumption, products intended for feeding animals (including pets), environmental samples in the area of food and feed production and handling, and all samples from the primary production stage
Published: 26/04/2024
Pages: 15
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and diluents
5.1 General
5.2 Diluents
5.3 Agar medium: plate count agar (PCA)
5.3.1 Composition
5.3.2 Preparation
5.3.3 Preparation of agar plates
5.3.4 Performance testing of the culture medium
5.3.4.1 General
5.3.4.2 Productivity
6 Apparatus
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.2.1
9.2.2
9.2.3
9.2.4
9.2.5
9.3 Counting of colonies
9.3.1
9.3.2
10 Expression of results
11 Test report
Annex A
A.1 General
A.2 Principle
A.3 Culture media and diluent
A.3.1
A.3.2
A.4 Apparatus
A.4.1
A.4.2
A.4.3
A.4.4
A.4.5
A.5 Sampling
A.6 Preparation of test sample
A.7 Procedure
A.7.1 Test portion and dilutions
A.7.2 Setting up
A.7.3 Inoculation
A.7.3.1 General
A.7.3.2 Sterility check
A.7.4 Incubation
A.7.5 Counting of colonies
A.7.5.1 Counting grid
A.7.5.2 Calibration and verification
A.7.5.3 Examination and reporting of spiral plate counts (manual method)
A.7.5.4 Examination, and reporting of spiral plate counts (using electronic colony counter)
A.8 Calculation and expression of results
A.9 Test report
Bibliography
Amendment 1
Corrigendum 1
Appendix ZZ
ZZ.1 Scope
ZZ.2 Modifications
Cited references in this standard
[Current]
Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media
[Current]
Microbiology of the food chain - General requirements and guidance for microbiological examinations
ISO 6887 (all parts)
Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination
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