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AS 5013.12.3-2004
[Current]Food microbiology, Method 12.3: Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Detection and MPN technique for low numbers
This Standard specifies a horizontal method for the enumeration and detection of coagulase-positive staphylococci, using the most probable number (MPN) technique. It is applicable to - products intended for human consumption and the feeding of animals, and - environmental samples in the area of food production and food handling. This method is recommended for products where staphylococci are expected to be stressed and in low numbers as, for example, in dried products.
Published: 13/07/2004
Pages: 11
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Detection method
4.1.1
4.1.2
4.1.3
4.1.4
4.1.5
4.1.6
4.1.7
4.2 Enumeration method
4.2.1
4.2.2
4.2.3
4.2.4
4.2.5
4.2.6
4.2.7
5 Diluents and culture media
5.1 Diluents
5.2 Modified Giolitti and Cantoni broth
5.2.1 Base medium
5.2.1.1 Composition
5.2.1.2 Preparation
5.2.2 Potassium tellurite solution
5.2.2.1 Composition
5.2.2.2 Preparation
5.2.3 Complete medium
5.2.4 Performance testing for the quality assurance of the culture medium
5.3 Agar solution (20 g/l)
5.3.1 Composition
5.3.2 Preparation
5.4 Baird-Parker agar medium
5.4.1 Composition and preparation
5.4.2 Performance testing for the quality assurance of the culture medium
5.5 Rabbit plasma fibrinogen agar medium (see references [6] and [7])
5.5.1 Composition and preparation
5.5.2 Performance testing for the quality assurance of the culture medium
5.6 Brain-heart infusion broth
5.6.1 Composition and preparation
5.6.2 Performance testing for the quality assurance of the culture medium
5.7 Rabbit plasma
6 Apparatus
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Detection method
9.1.1 Test portion and initial suspension
9.1.2 Enrichment
9.1.3 Subculture of tubes
9.2 Enumeration method
9.2.1 Test portion and initial suspension
9.2.2 Inoculation
9.2.3 Incubation
9.2.4 Subculture
9.3 Selection of plates and interpretation
9.3.1 Baird-Parker agar medium
9.3.1.1 Selection of colonies
9.3.1.2 Confirmation
9.3.2 Rabbit plasma fibrinogen agar medium
10 Expression of results
10.1 Detection method
10.2 Enumeration method
10.2.1 Selection of dilutions
10.2.2 Calculations
11 Precision
12 Test report
Bibliography
Cited references in this standard
Content history
[Current]
[Superseded]
DR 03634
AS 5013.12.3-2004 Rec:2016
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