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AS 5013.12.1-2004
[Current]Food microbiology, Method 12.1: Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — Technique using Baird-Parker agar medium
This Standard specifies a horizontal method for the enumeration of coagulase-positive staphylococci in products intended for human consumption or feeding of animals, by counting of colonies obtained on a solid medium (Baird-Parker medium) after aerobic incubation at 35 °C or 37 °C.
Published: 13/07/2004
Pages: 11
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1
4.2
4.3
5 Diluent and culture media
5.1 General
5.2 Diluent
5.3 Baird-Parker agar medium2)
5.3.1 Base medium
5.3.1.1 Composition
5.3.1.2 Preparation
5.3.2 Solutions
5.3.2.1 Potassium tellurite solution
5.3.2.1.1 Composition
5.3.2.1.2 Preparation
5.3.2.2 Egg yolk emulsion (concentration approximately 20 % or according to the manufacturer's instructions)
5.3.2.3 Sulfamezathine (sulfamethazine, sulfadimidine) solution
5.3.2.3.1 Composition
5.3.2.3.2 Preparation
5.3.3 Complete medium
5.3.3.1 Composition
5.3.3.2 Preparation
5.3.4 Preparation of agar plates
5.4 Brain-heart infusion broth
5.4.1 Composition
5.4.2 Preparation
5.5 Rabbit plasma
6 Apparatus and glassware
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation
9.2.1
9.2.2
9.2.3
9.3 Incubation
9.4 Selection of plates and interpretation
9.4.1
9.4.2
9.4.3
9.5 Confirmation (coagulase test)
10 Expression of results
10.1 General case
10.1.1 Calculation of the number a of coagulase-positive staphylococci identified for each plate selected
10.1.2 Calculation of the number N of identified coagulase-positive staphylococci present in the test portion
10.1.3 Example
10.2 Estimation of low numbers
10.2.1
10.2.2
11 Precision
12 Test report
Bibliography
AMENDMENT 1: Inclusion of precision data
Annex A
Cited references in this standard
Content history
[Superseded]
[Superseded]
ISO 6888-1:1999/Amd.1:2003
AS 5013.12.1-2004 Rec:2016
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