Standard
Track updates
AS 5013.11.1:2018
[Current]Food microbiology, Method 11.1: Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017, MOD)
The objective of this Standard is to define general rules for the aerobic preparation of the initial suspension and of dilutions for microbiological examination of products intended for human or animal consumption.
Published: 14/11/2018
Pages: 27
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents
5.1 Basic materials
5.2 Diluents for general use
5.2.1 Peptone salt solution
5.2.1.1 Composition
5.2.1.2 Preparation
5.2.2 Buffered peptone water
5.2.2.1 Composition
5.2.2.2 Preparation
5.2.3 Double-strength buffered peptone water
5.3 Diluents for special purposes
5.4 Distribution and sterilization of the diluent
5.5 Performance testing for diluents
6 Apparatus
6.1
6.2
6.2.1
6.2.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
6.11.1
6.11.2
6.11.3
6.11.4
6.11.5
6.11.6
6.12
6.13
6.14
7 Sampling
8 Preparation of samples
8.1 General
8.2 Frozen products
8.2.1 General
8.2.2 Small samples defrosted before testing
8.2.3 Large pieces or blocks sampled while frozen
8.2.3.1 General
8.2.3.2 Total sample (surface and depth)
8.2.3.3 Sample at depth only
8.2.3.4 Surface only sample
8.3 Hard and dry products
8.4 Dehydrated and other low-moisture products
8.5 Liquid and non-viscous products
8.6 Acidic products
8.7 High-fat (over 20 %) foods
8.8 Multi-component products
8.9 Packaged products
8.10 Surface samples (swabs and other devices)
9 Specific procedures
9.1 Test portion and initial suspension (primary dilution)
9.2 Duration of the procedure
9.3 Pooling and compositing procedures for qualitative tests
10 Further dilutions
10.1 Decimal dilution series
10.2 Other dilution series
Annex A
A.1 General
A.2 Composited samples
A.3 Pooled samples
A.4 Pooled test portions
A.5 Pooled (pre-)enriched test portions
Annex B
B.1 Non-homogeneous blocks
B.2 Homogeneous test pieces
Annex C
C.1 General
C.2 Dutch study comparing different sample sizes and effects of sample homogenization
C.3 French study comparing seven different sample preparation techniques
Annex D
D.1 Pooling tests
D.1.1 General
D.1.2 Inocula
D.1.3 Sample preparation
D.1.4 Pooling test portions
D.1.4.1 Preliminary reference test
D.1.4.2 Verification protocol
D.1.5 Pooling (pre-)enrichment portions
D.1.5.1 Preliminary reference test
D.1.5.2 Verification protocol
D.1.5.3 Interpretation of results
D.2 Confirmation of findings
Bibliography
Appendix ZZ
ZZ.1 Scope
ZZ.2 Variations
Cited references in this standard
[Current]
Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of culture media
[Current]
Microbiology of the food chain - General requirements and guidance for microbiological examinations
One-time Purchase
Access via web browser on any device
One-time purchase
Single publication
Offline access via PDF^
$141.91 AUD
Inclusive of GSTFormat *
Web Reader
Licenses *
1 user
Total$141.91 AUD
IMPORTANT