Standard
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AS 4276.5:2019
[Current]Water microbiology, Method 5: Coliforms, Escherichia coli and thermotolerant coliforms — Membrane filtration method
The objective of this Standard is to specify a method, using membrane filtration, for enumerating coliforms, Escherichia coli (E. coli) and thermotolerant coliforms in water other than packaged water.
Published: 07/03/2019
Pages: 24
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and culture media
6 Apparatus
7 Reference cultures
7.1 General
7.2 Positive cultures
7.3 Negative culture
8 Samples
8.1 Laboratory samples
8.2 Dilutions
9 Procedure
9.1 Filtration procedure
9.2 Incubation for combined total coliforms and E. coli enumeration
9.3 Resuscitation and incubation for combined E. coli and thermotolerant coliform enumeration
9.4 Identification of total coliforms and E. coli on MI agar
9.5 Identification of combined total coliforms and E. coli on chromogenic E. coli/coliform selective agar incubated at 36 °C ± 2 °C
9.6 Identification of combined E. coli and thermotolerant coliforms on chromogenic E. coli/coliform selective agar incubated at 44 °C ± 0.5 °C
9.7 Confirmatory tests
9.7.1 Coliforms
9.7.2 E. coli and thermotolerant coliforms
9.8 Interpretation of confirmatory results
9.8.1 Coliforms
9.8.2 E. coli
9.8.3 Thermotolerant coliforms
10 Calculation
11 Quality assurance
11.1 General
11.2 Performance testing of culture media
12 Test report
Appendix A
Appendix B
B.1 Scope
B.2 MI agar
B.2.1 Reference
B.2.2 Materials
B.2.3 Apparatus
B.2.4 Procedure
B.3 Chromogenic E. coli/coliform selective agar
B.3.1 Reference
B.3.2 Materials
B.3.3 Apparatus
B.3.4 Procedure
B.4 Nutrient agar
B.4.1 Reference
B.4.2 Materials
B.4.3 Apparatus
B.4.4 Procedure
B.5 Ortho-nitrophenyl-β-D-galactopyranoside (ONPG) broth
B.5.1 Reference
B.5.2 Materials
B.5.2.1 Solution 1 — Peptone water 1 %
B.5.2.1.1 Materials
B.5.2.1.2 Apparatus
B.5.2.1.3 Procedure
B.5.2.2 Solution 2 — ONPG solution
B.5.2.2.1 Materials
B.5.2.2.2 Apparatus
B.5.2.2.3 Procedure
B.5.2.3 Preparation of the complete medium — ONPG broth
B.5.2.3.1 Materials
B.5.2.3.2 Apparatus
B.5.2.3.3 Procedure
B.6 Oxidase reagent (Kovac’s oxidase reagent)
B.6.1 Reference
B.6.2 Materials
B.6.3 Apparatus
B.6.4 Procedure
B.7 EC-MUG broth
B.7.1 Reference
B.7.2 Materials
B.7.3 Apparatus
B.7.4 Procedure
Appendix C
Appendix D
Appendix E
E.1 General
E.2 Material
E.3 Apparatus
E.4 Procedure
E.5 Report
Bibliography
Cited references in this standard
Content history
[Superseded]
[Superseded]
DR AS 4276.5:2018
USEPA Method 1604
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