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AS 3753-2001

[Current]

Recommended practice for chemical analysis by ultraviolet/visible spectrophotometry

Sets out recommended procedures, principles and techniques involved in the setting up and operation of the UV/visible spectrophotometer for use in chemical methods of analysis.
Published: 05/05/2001
Pages: 25
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
1 Scope and general
1.1 Scope
1.2 Application
1.3 Principle
1.4 Referenced documents
1.5 Definitions
1.6 Apparatus
1.6.1 Volumetric glassware
1.7 Safety precautions
1.7.1 UV sources
1.7.2 Hazardous chemicals
1.7.3 Strong acids
1.7.4 Flammable solvents
1.7.5 Flow-through cells
2 Instrumentation
2.1 General
2.2 Sources of radiation
2.3 Wavelength selection
2.4 Optical system
2.4.1 General
2.4.2 Single-beam instrument
2.4.3 Double-beam instrument
2.4.4 Diode-array instrument
2.5 Measurement system
2.5.1 General
2.5.2 Detectors
2.5.3 Amplifier
2.5.4 Output device
2.6 Sample introduction
2.6.1 General
2.6.2 Cells
2.6.3 Cell material
2.6.4 Cell construction
2.6.5 Fibre optic dip probes
3 Optimization
3.1 Introduction
3.2 Cell care and handling
3.2.1 Cell handling
3.2.2 Cell cleaning
3.2.3 Test for cleanliness
3.2.4 Cell correction
3.2.5 Matched cells
3.2.6 Cell filling and emptying
3.2.7 Cell positioning
3.2.8 Cell storage
3.3 Monitoring spectrophotometer functions
3.3.1 Wavelength accuracy
3.3.1.1 Methods
3.3.1.2 Procedures
3.3.2 Stray radiation
3.3.3 Photometric accuracy
3.3.4 Zero absorbance line flatness
3.4 Factors affecting absorption
3.4.1 Solution characteristics
3.4.2 Cell characteristics
3.4.3 Refractive index
3.4.4 Reflection
3.4.5 Turbidity
3.5 Background absorption
3.6 Matrix effects
3.7 Setting up the instrument
3.8 Additional considerations
3.8.1 General
3.8.2 Spectral bandwidth
3.8.3 Absorption spectrum
3.8.4 Stability of the chromophore
4 Calibration and analysis
4.1 Reagent purity
4.2 Reference materials
4.3 Identification using wavelength scanning
4.3.1 Reference materials and spectra
4.3.2 Methodology
4.3.3 Procedure
4.4 Determinations at fixed wavelengths
4.4.1 Calibration solutions and graphs
4.4.1.1 General
4.4.1.2 Concentrations of calibration and test solutions
4.4.1.3 Calibration graphs
4.5 Calibration and test procedure
4.5.1 Absorbance measurements
4.6 Acceptance of results
4.6.1 General
4.6.2 Acceptance criteria
4.6.2.1 Single test sample
4.6.2.2 Replicate determinations
4.6.2.3 Acceptance of reference material results
Appendix A
Appendix B
Cited references in this standard
[Current]
Water for analytical laboratory use - Specification and test methods
[Current]
Filters for eye protectors, Part 2: Filters for protection against ultraviolet radiation
[Withdrawn]
Chemical analysis — Interlaboratory test programs — For determining precision of analytical method(s) — Guide to the planning and conduct
[Withdrawn]
Graduated straight pipettes
[Withdrawn]
Laboratory glassware — One-mark pipettes
Content history
[Superseded]
DR 00194
AS 3753-2001 Rec:2016

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