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AS 2300.1.10-2008

[Current]

Methods of chemical and physical testing for the dairying industry, Method 1.10: General methods and principles — Determination of phosphatase activity

This Standard sets out chemical tests for determining the amount of alkaline phosphatase enzyme (phosphomonoesterase) activity present in a sample of milk or milk product.
Published: 26/08/2008
Pages: 9
Table of contents
Cited references
Content history
Table of contents
Header
About this publication
Preface
1 Scope
2 Application
3 Referenced document
4 Principle
5 Reaction
6 Reagents
6.1 General requirements
6.2 Reagent solutions
7 Apparatus
8 General test precautions
9 Samples
9.1 Laboratory sample
9.2 Preparation of test samples
9.2.1 General
9.2.2 Liquid milk, buttermilk and whey
9.2.3 Cream
9.2.4 Cheese
9.2.5 Casein, caseinate and coprecipitate
9.2.6 Dried milk products (milk, buttermilk and whey)
10 Controls
10.1 Negative control
10.2 Positive control
11 Test procedures for products other than flavoured milks and cheese
11.1 Comparator method
11.2 Colorimetric (reference) method
12 Test procedure for flavoured milks
13 Test procedures for cheese
13.1 General
13.2 Colorimetric (reference) method
13.3 Spectrophotometric method
13.4 Preparation of p-nitrophenol standard solutions in cheese
14 Accuracy and uncertainty measurement
15 Report
Bibliography
Cited references in this standard
[Superseded]
Safety in laboratories, Part 2: Chemical aspects
[Withdrawn]
Milk and milk products — Guidance on sampling
Content history
[Superseded]
AS 2300.1.10-2008 REC:2019
DR 07346

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